Pac2 cell
WebMay 15, 2024 · Gene expression analysis using the PAC2-zGRβ cell line. The pCS2+zGRβ plasmid (Schaaf et al., 2008) and a neomycin resistance plasmid were transfected into PAC2 zebrafish cells using the Amaxa ® Cell line Nucleofector kit V and the Nucleofector™ II device (Lonza). Four days after transfection, cells were subjected to selection for ... WebMar 21, 2024 · GeneCards Summary for PACS2 Gene. PACS2 (Phosphofurin Acidic Cluster Sorting Protein 2) is a Protein Coding gene. Diseases associated with PACS2 include Developmental And Epileptic …
Pac2 cell
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WebA perovskite solar cell using the SAM hole transport layer can realize more than 20% efficiency without any dopants, and very cost effective because extremely low material … WebFeb 1, 2013 · The immunoblotting of zebrafish CD from PAC2 cells resolved by 2D electrophoresis confirmed that zebrafish mature CD exists as a singular molecular specie of approximately 41 kDa with a computed isoelectric point of about 5.4 (Fig. 2 A).To see whether the lack of the β-hairpin loop in the primary sequence of zebrafish CD indeed …
WebApr 8, 2013 · For cell-cycle analysis, PAC2 cells or dissociated embryos/larvae were fixed in cold 70% (vol/vol) ethanol, treated with 100 µg/mL RNase A (Sigma-Aldrich), and … WebAug 17, 2024 · Cells were seeded into white, clear-bottom 96-well plates (Corning, New York, USA) at a density of 10 4 cellls/well for Pac2, 1.25*10 4 cells/well for ZF4, and …
WebNov 13, 2024 · Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary according to species, including regulating morphological transition and virulence, mating and secondary metabolism. ... 4 Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, Brazil. WebMay 13, 2016 · Luciferase quantitation assays revealed that SbaP can drive luciferase gene expression in all eight cell lines including sf21 (insect), PAC2 (zebrafish), EPC (carp), CHSE-214 (chinook salmon), GSTEF (green sea turtle), MS-1 (monk seal), 293T (human), and HeLa (human), but at different levels.
WebThe PAC1–PAC2 heterodimer was originally identified in a search for proteasome-associated proteins in human cells 42. PAC1–PAC2 was found to be mainly associated with a proteasome assembly...
WebMar 4, 2010 · a Pac2 is stabilized in cells treated with the proteasome inhibitor MG132. pGAL-GFP-PAC2 transformed into pdr5Δ mutant cells was induced overnight with 2% galactose. Next morning the cells were diluted 1:3, and after 2 h 100 μl MG132 dissolved in DMSO was added (controls were treated with DMSO), together with 3% glucose to … furnivals pottery englandWebZEM2S cells were derived from ZEM2 in 1993 by selection for growth in a basal nutrient medium supplemented with 5 to 10% heat-inactivated fetal bovine serum. Comments … give cookies in cookie clickerWebJun 1, 2009 · The PAC2 fibroblast line, isolated from 24-h post-fertilization zebrafish embryos, is one of the few available lines. Here, we provide a basic set of methods for … give cookies cheatWebJul 1, 2008 · Pac2 cells, a fibroblastic cell line derived from zebrafish embryos, were a generous gift from Dr. Shawn Burgess (National Institutes of Health, Bethesda, MD, USA). They were maintained in Leibovitz's L-15 medium supplemented with 15% FBS and 1% penicillin/streptomycin at 32 °C without CO 2. Pac2 cells were split at a ratio of 1:10 ... give couch for cashWebFeb 10, 2024 · 3 lac system makes it a more adaptable tool for studying the temporal dynamics of gene expression, compared to constitutively active or repressed reporter … furnival\\u0027s innWebMIA PaCa-2 is an epithelial cell line that was derived from tumor tissue of the pancreas obtained from a 65-year-old, White male. This cell line can be used in cancer research. Product category Human cells Organism … furnival works addressWebIn constrast, PAC2 cells are capable of activating a promoterless neomycin gene at a frequency similar to that observed in cultured mouse cells. Analysis of the fusion transcript of one of the neomycin resistant PAC2 clones demonstrated that the neomycin resistance gene was activated by fusion with a gene normally expressed in PAC2 cells. furnival works