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Peasy basic seamless cloning and assembly kit

WebJul 16, 2024 · We are proud to announce the launching of pEASY®-Basic Seamless Cloning and Assembly Kit! This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments. WebDec 1, 2024 · Recently a commercially available pEASY -Uni Seamless Cloning and Assembly Kit (TransGen Biotech) has been made based on the technology of Gibson …

Evaluation of pEASY-Uni Seamless Cloning and Assembly Kit to …

Web5 rows · Product Details. This kit takes advantage of proprietary recombinases and homologous recombination ... WebCloning Vectors 1 Selected products (0/20) Contact supplier Click to select products that you want to inquire about before clicking the Contact supplier button. pEASY-Blunt3 Cloning Kit $92.30 / piece pEASY-T1 Cloning Kit $80.00 - $90.00 / set 1 set (Min. Order) pEASY-T1 Simple Cloning Kit $80.00 - $90.00 / set 1 set (Min. Order) how old are the boomerang generation https://melhorcodigo.com

A recombinant Mycobacterium smegmatis-based surface display …

WebpEASY® -Basic Seamless Cloning and Assembly Kit 目录号： CU201-02 单 价： ￥620 规格： 20 rxns 60 rxns 数量： - + 说明书 质检报告 立即下单 加入购物车 产品详情介绍 本产品 … WebOkegawa Y, Motohashi K. A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit. Biochem Biophys Rep 2015;4:148-151. Motohashi K. Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods. WebpEASY ®-Basic Seamless Cloning and Assembly Kit Cloning Competent Cells Trans 10 Chemically Competent Cell Trans 5α Chemically Competent Cell Trans 109 Chemically Competent Cell Trans 110 Chemically Competent Cell Trans 1-Blue Chemically Competent Cell Trans 2-Blue Chemically Competent Cell Trans 1-T1 Phage Resistant Chemically … mercedes glc300 reviews

pEASY-Uni Seamless Cloning and DNA Assembly Kit – …

Category:Evaluation of pEASY-Uni Seamless Cloning and Assembly Kit to …

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Peasy basic seamless cloning and assembly kit

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WebThe four amplified fragments were gel purified and assembled into pBac[3×P3-EGFP afm] digested with QuickCut TM BglII using pEASY ®-Basic SeamLess Cloning and Assembly Kit; the 4000-bp genomic fragment was used as the promoter of AsOBP1 gene, and AsOBP1 and EGFP were fused through the T2A linker. The OE vector was classified as pBac[AsOBP1 ... WebPART 1: Protocols （1）Molecular cloning： 【1】PCR Primers were designed by snapgene sotware and synthesized at GENEWIZ Company. PCR was performed by following the manuals of PrimeSTAR Max Premix (2X) (Taraka, R045) or 2 X EasyTaq PCR SuperMix (+ dye) (Transgene, M256).

Peasy basic seamless cloning and assembly kit

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WebApr 12, 2024 · The subsequent ligation reaction was carried out at 50 °C according to the pEASY-Basic Seamless Cloning and Assembly Kit (TransGen Biotech), after which ligation products were transformed into E. coli DH5α. Positive colonies were screened and plasmid was isolated and sent to Sangon (Shanghai, China) for DNA sequencing. Protein purification WebThe GeneArt™ Seamless Cloning and Assembly Kit enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any …

WebSorry, we have detected unusual traffic from your network. Please slide to verify. Click to feedback > WebTransGen Biotech pEASY®-Basic Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional …

WebFast accurate construct design for all major molecular cloning techniques; Validate sequenced constructs using powerful alignment tools; Customize plasmid maps with … WebMay 17, 2016 · The PQQ and terminal ubiquinol cytochrome bo 3 oxidase fused expression plasmid was constructed by the pEASY-Uni seamless cloning and assembly kit CU101 (TransGen, China). ... The cDNA was synthesized from the total RNA using a PrimeScript RT Reagent Kit (Perfect Real Time) (Takara) according to the manufacturer’s protocol.

WebJan 12, 2024 · Expression vectors were constructed using pEASY® -Basic Seamless Cloning and Assembly Kit (TransGen Biotech, Beijing, China). The whole coding sequences were amplified from cDNA templates of young fruit of ‘Xindajiubao’ using the KOD-plus-neo high-fidelity enzyme kit (Toyobo, Osaka, Japan). mercedes glc 300 seat coversWebSeamless cloning was performed using pEASY-Basic Seamless Cloning and Assembly Kit (Transgen). Assembly of recombinant vectors Plasmid pI1818 was constructed for surface display in BCG and ZWY2 under the control of the strong mycobacterium hsp60 promoter followed by an N-terminal truncated Rv1818’ , a transportation protein anchored in the ... how old are the boomer generationWeb5 rows · Sep 12, 2024 · Features of the pEASY-Basic CU201. Fast assembly and fast cloning: 15 minutes. Easy: ... how old are the botched doctorsWebMay 9, 2024 · To develop the plasmids expressing ASFV p32 and p54, we constructed the replicon plasmids pSFV-p32 and pSFV-p54 via homologous recombination using the pEASY ® -Basic Seamless Cloning and Assembly Kit and pSFV-EGFP as described above. mercedes glc 300 running boardsWebFeatures of the pEASY-Uni Seamless Cloning and Assembly Kit Fast assembly and fast cloning: 15 minutes Easy: regardless of restriction enzyme sites of inserts, and … mercedes glc300 reviews 2022WebGibson was performed by following the manual of pEASY- Basic Seamless Cloning and Assembly Kit (Transgene, CU201). 【4】Endonuclease digestion and ligation The endonucleases and T4 ligase were bought from NEB Company and the experiments were performed by following instructions. 【5】Transformation how old are the brady boysWebTransGen Biotech pEASY® -Uni Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional … mercedes glc 300 reviews 2021