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Phis2 载体

Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a … http://www.youbio.cn/product/vt1637

phis2.1载体图 pHis2酵母单杂交载体 - 无锡文化网

Webb质粒简介:. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple ... http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html russian taxi game show https://melhorcodigo.com

pYES2载体图谱质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体

http://muchong.com/t-9775583-1 http://www.kelei-biology.com/plus/view.php?aid=1071 Webb使用pHIS2载体,将启动子序列构建在HIS3报告基因前面的多克隆位点上,对应的转录因子构建在AD载体上即可。 使用Y187菌株,它相对于AH109菌株在基因组上少了HIS的报告 … russian tape recorder

中国科学院植物研究所酵母单杂交文库筛选客户案列展示 - 知乎

Category:phis2酵母表达载体说明 - 豆丁网

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Phis2 载体

pYES2说明书-分析方法-生物在线 Lab-on-Web

Webb11 juli 2014 · 常用载体及通用测序引物.docx 2014-07-11 上传 常用载体及通用测序引物,测序通用引物,华大测序通用引物,载体通用引物,petduet 1测序引物,通用引物,测序引物,pet28a测序引物,m13通用引物,细菌通用引物,病毒通用引物 http://www.bioon.com.cn/doc/showarticle.asp?newsid=97329

Phis2 载体

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http://zomanbio.com/shengwu_info.php?nid=2549 WebbpHIS-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …

Webb1.构建载体目的基因融合连入pGADT7-Rec2(AD); 顺式作用元件连入pHis2或pHis2.1载体(顺式元件可以只连接一个拷贝, 也可以连接3个串联的并测序确认无误)。 2.验证两载体分别于对应的空载体共转时,有无激活转录报告基因情况,大多酵母苗株有毒性。 WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, …

http://www.bioon.com.cn/reagent/show_product.asp?id=3900880 Webb26 maj 2024 · 载体描述 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically …

Webb载体名称: pHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点,限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: …

Webb6 sep. 2024 · 答: 有3种常用的最基本启动子,分别为pHis2载体的PminHIS3启动子,报告基因为HIS3;pLacZ载体的PCYC1启动子,报告基因为LacZ;pAbAi载体的iso-1 … schedule for presidential debatesWebbpAbAi载体价格1500元,pAbAi载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为氨苄青霉素,质粒大小为4894 bp。pAbAi载体是酵母单杂交系统。 schedule for provision for doubtful debtshttp://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html russian tape in hair extensionsWebb12 apr. 2024 · 方法:以启动子A构建到酵母单杂交启动子载体pHIS2,文库质粒为pGADT7-中山杉文库。 材料: 1.启动子克隆:pHIS2-A。 根据实验需求进行扩增并酶切构建载体,两端的EcoRI和 SacI酶切位点,序列如下: GAATTCAGTTGGAATCACATCTATCATCAGAATGAAAGACCTAAGAAAAAAATGGCA … russian tank tree war thunderWebb12 apr. 2024 · 膜上载体蛋白属内在蛋白,它有选择地和膜一侧的分子或离子结合,形成载体-物质复合物,通过构象变化透过膜,把分子或离子释放到另一侧。 载体蛋白三种类型 . 1、单向运输载体:催化分子或离子单方向跨膜运输,顺电化学势差进行。 russian tattoo meanings and placementWebb载体应用. To use pHIS2 in a one-hybrid assay, clone one or more copies of a cis-acting DNA target sequence into the MCS. Then introduce the plasmid into competent yeast cells using the transformation protocol in the BD Matchmaker Library Construction & Screening Kits User Manual (PT3529-1). schedule for osu men\u0027s basketballhttp://www.labbase.net/Supply/SupplyItems-4229347.html schedule for queen\u0027s funeral