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Prohead protease

WebAuthors confirm that the catalytic activity resides in the N-terminal two-thirds of the protease polypeptide and suggest that the C-terminal one-fifth of the protein contains a capsid targeting signal. PMID: 23688818; Subcellular Location: Virion. Protein Families: HK97 prohead protease protein family: Database Links: KEGG: vg:1262531 WebEach of these three groups will be considered in turn by focusing on the following systems: the Rec A-dependent intramolecular cleavage of the Escherichia coli SOS response protein, LexA; the intramolecular activation of the mammalian aspartic acid protease, pepsinogen; and the autocatalytic cleavage of polyproteins synthesized by picornaviruses.

Experimental evidence for proteins constituting virion components …

Webcapsid maturation protease Protease, prohead protease Sisi_4 major capsid protein capsid Sisi_6 head-to-tail connector protein Sisi_7,8,9,10 major tail protein major tail subunit Sisi_11 tail assembly chaperone Tail scaffolding protein TM4_15; 16 tape measure protein Tape Measure, tmp, tapemeasure TM4_17 WebUniProtKB reviewed (Swiss-Prot) Organism. Enterobacteria phage HK97 (Bacteriophage HK97) Amino acids. 225. Protein existence. Evidence at protein level. Annotation score. 2/5. ladda ner office 2021 professional plus https://melhorcodigo.com

Common Evolutionary Origin of Procapsid Proteases

WebJun 26, 2024 · Phage G's decoration protein is longer than what has been reported in other phages, and we suspect the extra interaction surface area helps stabilize the capsid. We identified several additional capsid proteins, including a candidate for the prohead protease responsible for processing gp27. WebJan 1, 2005 · If proheads are assembled with a mutant form of the protease that is catalytically inactive but competent for assembly, there are roughly 50 molecules of protease per prohead, and this is presumed to be the same as the number of wild‐type proteases per prohead. WebMar 20, 2012 · The identification of 39 cleavage sites in 19 φKZ head proteins indicates cleavage of many prohead proteins forms a major morphogenetic step in φKZ head … proper sheep handling

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Prohead protease

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WebCaudovirus prohead serine protease. Family of Caudovirus prohead serine proteases also found in a number of bacteria possibly as the result of horizontal transfer. Links. WebJul 5, 2024 · Proteomic analyses of gp245- proheads that the normal proteolytic processing of head proteins had not occurred. Assays of a recombinant, truncated form of the …

Prohead protease

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WebAug 9, 2013 · Proheads were made and purified using plasmids that express the HK97 major capsid protein gp5 and either wild-type protease or gpD–protease fusion proteins made using several of the protease linker insertion mutants described in the text. The relative size and gp4 content of each is indicated in the figure. WebCleavage; by prohead core protease GP21: Sequence: EA: Expand table. GO annotations. Aspect Term; Cellular Component: virion component Source:UniProtKB-KW. Expand table. Complete GO annotation on QuickGO . Names & Taxonomy. Protein names. Recommended name. Prohead assembly protein gp68. Alternative names. Gene product 68 (gp68) …

WebMay 17, 2013 · Prohead-1 To overcome the transient nature of Prohead-1 in the presence of the viral protease, we engineered an inactivating point mutant of the protease catalytic histidine (H65A),... WebAug 1, 2004 · Most of the known prohead maturation proteases in double-stranded-DNA bacteriophages are shown, by computational methods, to fall into two evolutionarily …

WebMay 17, 2013 · The HK97 protease is predicted (i.e., by PSIPRED 47) to have a set of C-terminal α-helices in the last ~60 residues that may be configured to interact with the delta domain of the HK97 major capsid protein, and thereby direct the assembly of the protease into the HK97 prohead. The two partners of this interaction may both be alpha-helical. WebThe prohead is then released from the host membrane and undergoes the final step of head assembly—DNA packaging where the terminase proteins packages the 170 kb genome …

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WebNov 1, 2016 · The prohead has an internal core, made of scaffolding proteins, and an outer shell, formed by the major capsid protein. The prohead usually contains a protease, which … proper shedsladda ner ms office pro plus 2021 gratisWebJun 26, 2024 · Mass spectrometry analysis of phage G identifies (1) all major capsid components and (2) the prohead protease responsible for the processing of the major capsid protein, gp27. • Phage G structural studies led to proteomic and genomic analyses that revised the identity of the phage G host from Bacillus megaterium to a Lysinibacillus … ladda ner office 365 familyWebApr 7, 2004 · The prohead assembly is initiated by the portal protein gp20. The prohead contains an internal core made up of the major core protein, gp22, the minor core … ladda ner office 2010 gratisWebThe procapsid of the Bacillus subtilis bacteriophage SPP1 is formed by the major capsid protein gp13, the scaffolding protein gp11, the portal protein gp6, and the accessory protein gp7. The protein stoichiometry suggests a T = 7 symmetry for the SPP1 procapsid. Overexpression of SPP1 procapsid proteins in proper shine detailingWebJan 1, 1986 · The T4 prohead protease (T4 Pase) is the key enzyme in the morphopoietic pathway of the T4 phage head. It is responsible for the proteolytic processing of all head … ladda ner office 365 homeWebMar 30, 2024 · Interestingly, the prohead protease and the MCP pVOG genes are very close to the bS21 gene (generally 2–4 genes; Fig. 7b), as in the bS21-encoding phage genomes analyzed in this study (2–6 ... ladda ner office outlook